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KMID : 1143420180110341125
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Public Health Weekly Report
2018 Volume.11 No. 34 p.1125 ~ p.1129
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Construction of luminescence and fluorescence tagged Burkholderia pseudomallei for pathogen tracking in a mouse model
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Shin Yong-Woo
Park Deok-Bum
Choi Myung-Min
Chun Jeong-Hoon
Rhie Gi-Eun
Seong Baik-Lin
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Abstract
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Molecular imaging of pathogens causing infectious diseases, at both cell and whole organism levels, is crucial to studying the pathogenesis mechanisms of infectious diseases. In this study, we have produced a luminescence and fluorescence duallabeled form of Burkholderia pseudomallei , a high-risk pathogen, which can be used to study pathogenesis and the immune responses at live organism, isolated organ, tissue and cell levels simultaneously. Using this method, we could trace B. pseudomallei in mice, and visualize bacterial replication in cells. The method is highly applicable to other pathogens, and could be an extremely useful tool to study the pathogenesis and host immune responses of a variety of infectious diseases.
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KEYWORD
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Burkholderia pseudomallei , Molecular imaging, Luminescence, Fluorescence
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